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mouse monoclonal anti rad21  (Novus Biologicals)


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    Novus Biologicals mouse monoclonal anti rad21
    Mouse Monoclonal Anti Rad21, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti rad21/product/Novus Biologicals
    Average 91 stars, based on 1 article reviews
    mouse monoclonal anti rad21 - by Bioz Stars, 2026-02
    91/100 stars

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    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
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    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
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    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
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    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
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    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of <t>RAD21,</t> in these four samples. Gray dots often overlap with black dots. .
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    siRNA and Primers in This Study
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    Image Search Results


    ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

    Journal: EMBO Reports

    Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

    doi: 10.1038/s44319-024-00303-6

    Figure Lengend Snippet: ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

    Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

    Techniques: Flow Cytometry, Immunoprecipitation, Western Blot

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

    doi: 10.1038/s44319-024-00303-6

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

    Techniques: Recombinant, Sequencing, Reverse Transcription, SYBR Green Assay, Software, Isolation

    siRNA and Primers in This Study

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: cccDNA Surrogate MC-HBV–Based Screen Identifies Cohesin Complex as a Novel HBV Restriction Factor

    doi: 10.1016/j.jcmgh.2022.08.002

    Figure Lengend Snippet: siRNA and Primers in This Study

    Article Snippet: The commercial antibodies used for Western blot in this study were as follows: rabbit anti-SMC3 monoclonal antibody (1:2000, 5696; Cell Signaling Technology, Danvers, MA), rabbit anti-SMC1A monoclonal antibody (1:10,000, ab109238; Abcam, Cambridge, MA), rabbit anti-CTCF polyclonal antibody (1:4000, ab188408; Abcam), rabbit anti-HBc polyclonal antibody (1:2000, B0586; Dako, Copenhagen, Denmark), rabbit anti-HBx polyclonal antibody (1:2000, ab39716; Abcam), mouse anti-PDS5B monoclonal antibody (1:50, SC-81635; Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-PDS5A monoclonal antibody (1:50, SC-515263; Santa Cruz Biotechnology), mouse anti-RAD21 monoclonal antibody (1:50, SC-271601; Santa Cruz Biotechnology), mouse anti–β-actin monoclonal antibody (1:5000, 66009-I-Ig; Proteintech, Chicago, IL), mouse anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) monoclonal antibody (1:5000, 66004-I-Ig; Proteintech), mouse anti-Flag-tag monoclonal antibody (1:1000, M2; Sigma), and mouse anti-HA-tag monoclonal antibody (1:2000, ab130275; Abcam).

    Techniques: Sequencing